30 March 1995 Imaging microtubules in buffer solution using tapping mode atomic force microscopy
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Taxol stabilized microtubules were imaged in their native state in buffer solution without any fixation by an atomic force microscope (AFM) operated in tapping mode in liquids. Glass cover slips were derivatized with a positively charged silane to adsorb the filaments. The adsorbed microtubules could be imaged stably without any visible damage for hours. In cases where a microtubule crossed above another one, it often broke to follow the curvature of the underlying one. Longitudinal structures with a spacing of about 8 nm could be resolved suggesting they are protofilaments.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Monika Fritz, Monika Fritz, Manfred Radmacher, Manfred Radmacher, Miriam W. Allersma, Miriam W. Allersma, Jason P. Cleveland, Jason P. Cleveland, Russell J. Stewart, Russell J. Stewart, Paul K. Hansma, Paul K. Hansma, Christoph F. Schmidt, Christoph F. Schmidt, } "Imaging microtubules in buffer solution using tapping mode atomic force microscopy", Proc. SPIE 2384, Scanning Probe Microscopies III, (30 March 1995); doi: 10.1117/12.205923; https://doi.org/10.1117/12.205923


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