3 April 1995 Ultrahigh-speed DNA sequencing using capillary array electrophoresis chips
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Abstract
Capillary electrophoresis arrays have been fabricated on planar glass substrates using photolithographic masking and chemical etching. The photolithographically defined channel patterns were first etched in a glass substrate, and then capillaries were formed by thermally bonding the etched substrate to a second glass slide. High-speed separations of restriction fragment digests were performed on these chips in under 1 minute. Multiple separations in the same channel demonstrated excellent reproducibility. Rapid genetic typing of short tandem repeats on the HUMTHO1 locus have also been performed. Finally, separations with single- base resolution have been successful, indicating that these microfabricated devices will also be useful for high-speed DNA sequencing.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Adam T. Woolley, Richard A. Mathies, "Ultrahigh-speed DNA sequencing using capillary array electrophoresis chips", Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); doi: 10.1117/12.206006; https://doi.org/10.1117/12.206006
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