Analysis of downregulation of cellular energy demand by 2D measurements of intracapillary HbO2, Hb, pO2, and redox state of cytochromes

Alfons Krug; Manfred D. Kessler; Jens Hoeper; S. Zellner; Valerie Sourdoulaud

doi:10.1117/12.206830

12 April 1995 Analysis of downregulation of cellular energy demand by 2D measurements of intracapillary HbO₂, Hb, pO₂, and redox state of cytochromes

Alfons Krug, Manfred D. Kessler, Jens Hoeper, S. Zellner, Valerie Sourdoulaud

Author Affiliations +

Proceedings Volume 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II; (1995) https://doi.org/10.1117/12.206830
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Rapid microlightguide spectrometers (EMPHO IIa/b) and a multiwire pO₂ electrode are applied for measurements of heterogeneous distribution of tissue oxygenation and redox state of respiratory enzymes in heart and rat liver. Optical and pO₂ measurements are noninvasively performed by use of sensors placed on the surface of tissue. Measurements in isolated perfused rat and in dog heart in situ were performed in order to investigate the relation between myocardial oxygenation and function. The tissue monitoring in liver was initiated by optical and polarographic monitoring in the hemoglobin free perfused organ. Subsequently, erythrocytes were added to the perfusate in several steps. The experiments reveal clear evidence that a protective system of tissue is activated when critical pO₂ values at the lethal corner of micro vessels fall off a critical threshold around 5 mmHg, thus causing a depletion of oxidative metabolism.

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Alfons Krug, Manfred D. Kessler, Jens Hoeper, S. Zellner, and Valerie Sourdoulaud "Analysis of downregulation of cellular energy demand by 2D measurements of intracapillary HbO₂, Hb, pO₂, and redox state of cytochromes", Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); https://doi.org/10.1117/12.206830

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