12 April 1995 Mechanisms and limits of contrast in optical imaging of cancer
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The mechanisms of optical contrast in biological tissues and the limits of contrast are discussed. Some cancers develop as cellular masses which displace normal fibrous tissue or stroma, yielding a net drop in scattering properties. Some cancer may be more fibrous than normal tissues yielding a net increase in scattering. Cancers sometimes develop increased vascularity which yields a net increase in absorption. Another measure is fluorescence, either autofluorescence or dye-enhanced fluorescence. This paper considers the limits of resolution when optically characterizing a small spherical object within a turbid tissue. The ability to discriminate a small object with a strongly increased absorption vs a larger object with a slight increase in absorption is tested, using frequency-domain light transport with 3-GHz modulation of the light source. The results suggest that an object about 1/10 the size of the tissue within which it is embedded can be distinguished by a pair of measurements: the phase difference (Delta) P and the amplitude ratio A/A0.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Steven L. Jacques, Steven L. Jacques, } "Mechanisms and limits of contrast in optical imaging of cancer", Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); doi: 10.1117/12.206808; https://doi.org/10.1117/12.206808

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