Noninvasive redox and back-scattered light imaging of keratocyte cells in the cornea: two-photon excitation and scanning slit confocal microscopy

Barry R. Masters

doi:10.1117/12.206831

12 April 1995 Noninvasive redox and back-scattered light imaging of keratocyte cells in the cornea: two-photon excitation and scanning slit confocal microscopy

Barry R. Masters

Author Affiliations +

Proceedings Volume 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II; (1995) https://doi.org/10.1117/12.206831
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

The ability to image and monitor the metabolic activity of keratocytes is important for the investigation of wound healing and repair mechanisms in the cornea. After laser refractive surgery there is activation of the stromal keratocytes in the human cornea. Two-photon excitation laser scanning microscopy was used to monitor the NAD(P)H levels in keratocytes in the cornea. The autofluorescence was confirmed to be mostly of NAD(P)H origin by treatment with cyanide which caused an increase in the fluorescence by a factor of two. We used a real-time scanning slit confocal microscope to image the distribution of keratocytes in the full thickness of the cornea. This microscope has the ability to image the cellular processes as well as the nuclei of the stromal keratocytes. Noninvasive optical imaging may provide a useful tool to investigate keratocyte activation after laser surgery or wound healing.

Citation Download Citation

Barry R. Masters "Noninvasive redox and back-scattered light imaging of keratocyte cells in the cornea: two-photon excitation and scanning slit confocal microscopy", Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); https://doi.org/10.1117/12.206831

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
15 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Cornea

Confocal microscopy

Microscopes

Cell mechanics

Luminescence

Eye

In vivo imaging

Show All Keywords

Keywords/Phrases

Search In:

Publication Years