Probability description of fluorescent and phosphorescent signal generation in tissues and other random media

Eva Marie Sevick-Muraca; Christina L. Hutchinson

doi:10.1117/12.206805

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12 April 1995 Probability description of fluorescent and phosphorescent signal generation in tissues and other random media

Eva Marie Sevick-Muraca, Christina L. Hutchinson

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Proceedings Volume 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II; (1995) https://doi.org/10.1117/12.206805
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

The origin of fluorescent or phosphorescent signals re-emitted from highly scattering media (such as tissues) is investigated using a simple probability analysis developed earlier by Seveck et al. and adapted for incorporation of excited state probe kinetics. Results show that the lifetime of a uniformly distributed phosphorescent or fluorescent optical probe will profoundly affect the volume interrogated by a noninvasive, reflectance measurements. When the lifetime is greater than photon migration times, the origin of the re-emitted signal is confined closest to the surface. Our computations suggest that noninvasive measurements of tissue oxygen concentration may not necessarily interrogate deep tissues when systemically administered phosphorescent dyes are used. Instead, our results point to the development of short- lived probes and the successful deconvolution of nanosecond photon migration times for sensing subsurface conditions.

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Eva Marie Sevick-Muraca and Christina L. Hutchinson "Probability description of fluorescent and phosphorescent signal generation in tissues and other random media", Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); https://doi.org/10.1117/12.206805

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