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12 April 1995 Three-dimensional confocal fluorescence microscopic visualization of the living human skin
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Three-dimensional confocal visualization of living human skin is a new development in the noninvasive imaging of normal and pathological tissue. I have investigated the autofluorescence of in vivo human skin with a laser scanning confocal microscope. An argon ion laser (488 nm) was used for excitation of the natural fluorescence of skin and a 515 nm cut off filter was used to separate the fluorescence from the excitation light. I found that normal skin has a very high autofluorescence. The laser scanning confocal microscope was used to obtain a stack of serial sections through the skin. A stack of optical sections through the hair follicle was reconstructed as well as the three-dimensional reconstruction of the pores of sweat glands. The ability to obtain two and three-dimensional visualizations of in vivo human skin may provide a new tool for noninvasive diagnostics in dermatology.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Barry R. Masters "Three-dimensional confocal fluorescence microscopic visualization of the living human skin", Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); doi: 10.1117/12.206820;

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