Detection and characterization of human tissue lesions with near-infrared Raman spectroscopy

Michael S. Feld; Ramasamy Manoharan; Juha Salenius; Jacobo Orenstein-Carndona; Tjeerd J. Roemer; James F. Brennan III; Ramachandra R. Dasari; Yang Wang

doi:10.1117/12.208468

8 May 1995 Detection and characterization of human tissue lesions with near-infrared Raman spectroscopy

Michael S. Feld, Ramasamy Manoharan, Juha Salenius, Jacobo Orenstein-Carndona, Tjeerd J. Roemer, James F. Brennan III, Ramachandra R. Dasari, Yang Wang

Author Affiliations +

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208468
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Near infrared (NIR) Raman spectroscopy provides a powerful method for quantitative histochemistry of human tissue and disease diagnosis. The feasibility and potential of this technique for in situ histochemical analysis of human coronary artery has been demonstrated and presented in other reports from our laboratory. In this work, we review recent results obtained with the NIR Raman spectroscopy on a variety of tissue types studied at the MIT Laser Biomedical Research Center. We have collected NIR Raman spectra from colon, bladder, breast, and carotid artery. For colon, bladder and breast, consistent differences between carcinoma and normal tissue spectra were observed. For colon and bladder, the spectral differences appear to be due to an increased content of nucleic acid in carcinomas, while the spectral changes in malignant breast tissue are associated with an increase of protein content. Spectra from carotid artery have similar features as those from aorta and coronary arteries. We also show some preliminary results obtained with a NIR Raman microspectroscopy setup with 20 micron lateral resolution. The biochemical distributions for normal and diseased regions on the same tissue samples are observed. The potential of using this NIR Raman spectroscopy for detection and characterization of carcinoma and atherosclerosis, is discussed.

Citation Download Citation

Michael S. Feld, Ramasamy Manoharan, Juha Salenius, Jacobo Orenstein-Carndona, Tjeerd J. Roemer, James F. Brennan III, Ramachandra R. Dasari, and Yang Wang "Detection and characterization of human tissue lesions with near-infrared Raman spectroscopy", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208468

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available