Fiber optic evanescent wave spectroscopy (FEWS) and its applications for multicomponent analysis of blood and biological fluids

Ronit Simhi; David Bunimovich; Ben-Ami Sela; Abraham Katzir

doi:10.1117/12.208526

8 May 1995 Fiber optic evanescent wave spectroscopy (FEWS) and its applications for multicomponent analysis of blood and biological fluids

Ronit Simhi, David Bunimovich, Ben-Ami Sela, Abraham Katzir

Author Affiliations +

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208526
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

In numerous cases the technique of Fiberoptic Evanescent Wave Spectroscopy (FEWS) offers great advantages over regular IR spectroscopic methods. It provides an easy way for measuring the absorption spectra of highly absorbing or highly scattering samples. With FEWS one can perform measurements in situ and in real time and this is potentially useful for measurements on biological samples or for the monitoring of chemical reactions. In the present work, the FEWS technique was used to analyze human blood serum using a Fourier Transform Infrared Spectrometer. A special cell based on IR transmitting non-toxic silver halide fibers was designed. Further improvements in the analysis were obtained by adopting some multivariate calibration techniques that have already been used in clinical chemistry. The blood constituents analyzed were: urea, total protein, cholesterol, uric acid and calcium. Good agreement between our results and the ordinary chemical and enzymatic methods was obtained.

Citation Download Citation

Ronit Simhi, David Bunimovich, Ben-Ami Sela, and Abraham Katzir "Fiber optic evanescent wave spectroscopy (FEWS) and its applications for multicomponent analysis of blood and biological fluids", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208526

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