Immunoassays for pesticide monitoring

Ingrid Wengatz; Ferenc Szurdoki; Anand R. Swamy; Lawrence Evans III; Gabor Patonay; Eric Stimmann; Michael Delwiche; Donald Stoutamire; Shirley J. Gee; Bruce D. Hammock

doi:10.1117/12.208500

8 May 1995 Immunoassays for pesticide monitoring

Ingrid Wengatz, Ferenc Szurdoki, Anand R. Swamy, Lawrence Evans III, Gabor Patonay, Eric Stimmann, Michael Delwiche, Donald Stoutamire, Shirley J. Gee, Bruce D. Hammock

Author Affiliations +

Proceedings Volume 2388, Advances in Fluorescence Sensing Technology II; (1995) https://doi.org/10.1117/12.208500
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

This study compares two formats of rapid assays for the detection of pesticides (bromacil and pyrethroid based metabolites): enzyme linked immunosorbent assay (ELISA) and immunoassay with near-infrared (NIR) fluorescence detection. NIR dye immunoassay (NIRDIA) measurements were carried out by using two different instruments, both having a silicon photodiode as the detector and a laser diode for excitation. ELISA and NIRDIA were performed in a tracer format, where the specific antibody is bound to the surface of a microtiter plate well and the tracer with enzyme or fluorescent dye label competes with the analyte for the antibody binding site. It was demonstrated that the NIRDIA is at least as sensitive as the ELISA. Both assays detect pesticides in the (mu) g/L (ppb) range. Hapten- macromolecule-NIR dye-conjugates have been synthesized with various biopolymers (e.g., proteins) as carriers. The use of carrier macromolecules enables convenient purification of the cyanine dye derivatives. The mild conjugation method of the dye is based on isothiocyanate chemistry.

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Ingrid Wengatz, Ferenc Szurdoki, Anand R. Swamy, Lawrence Evans III, Gabor Patonay, Eric Stimmann, Michael Delwiche, Donald Stoutamire, Shirley J. Gee, and Bruce D. Hammock "Immunoassays for pesticide monitoring", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208500

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