8 May 1995 Lifetime-based identification of single molecules
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Abstract
Our new developed time-correlated single photon counting electronics allows for continuous registration of time-resolved fluorescence signals in millisecond intervals. With this electronics, adapted fluorescence measurement system, we studied single molecule transitions through a focused laser beam. In our paper we present measurements on single rhodamine 6G- molecules and discuss data processing methods, using the full information of the time resolved measurement. The use of this information influences significantly the reliability of the detection of single molecule transits. Further developments and applications of this method are discussed.
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Joerg Enderlein, Joerg Enderlein, Rolf Krahl, Rolf Krahl, Uwe Ortmann, Uwe Ortmann, Michael Wahl, Michael Wahl, Rainer Erdmann, Rainer Erdmann, Edgar O. Klose, Edgar O. Klose, } "Lifetime-based identification of single molecules", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208465; https://doi.org/10.1117/12.208465
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