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8 May 1995 Lifetime-based sensing in highly scattering media
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Abstract
Fluorescence lifetime-based sensing is now recognized as a valuable methodology in clinical and analytical chemistry. For clinical chemistry, or for non-invasive sensing through skin, it is often necessary to obtain quantitative information in highly scattering media. Lifetime-based sensing can be used to obtain quantitative measurements in turbid media. We describe frequency-domain lifetime measurements in intralipid suspension, and present a reliable technique which allows phase-modulation lifetime measurements of fluorophores dispersed within or localized within intralipid. Lifetimes can be measured using an intensity decay law which accounts for the time delays and pulse-broadening effects of multiple light scattering events occurring in the intralipid. Alternatively, the phase and modulation measurements can be performed relative to a reference fluorophore of known lifetime. This approach provided reliable lifetime data for a pH-sensitive fluorophore contained within a microcuvette 4 mm under the surface of an intralipid suspension.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Henryk Szmacinski and Joseph R. Lakowicz "Lifetime-based sensing in highly scattering media", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); https://doi.org/10.1117/12.208549
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