8 May 1995 Two-dimensional infrared fluorescence scanner used for DNA analysis
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Abstract
A LI-COR Model 4000 DNA Sequencer has been modified by removing the internal scanning infrared fluorescence microscope and combining it with an external, orthogonal scanner. Due to the reduced background fluorescence and light scattering of nylon membranes in the near- infrared (8000 nm) as compared to the visible region of the optical spectrum, sensitivity of labeled DNA fragments is enhanced. Dot blots of dilution series of labeled oligonucleotides reveal a detection limit of 25 attomole (25 X 10-18 mole). DNA fragments blotted onto nylon membranes using direct transfer electrophoresis in multiplex DNA sequencing can also be detected and subsequently analyzed.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Lyle Richard Middendorf, Lyle Richard Middendorf, Robert C. Bruce, Robert C. Bruce, John A. Brumbaugh, John A. Brumbaugh, Daniel L. Grone, Daniel L. Grone, Gi Y. Jang, Gi Y. Jang, Peter Richterich, Peter Richterich, Holges Joachim Hoeltke, Holges Joachim Hoeltke, Richard J. Williams, Richard J. Williams, Mauro Dr. Peralta, Mauro Dr. Peralta, } "Two-dimensional infrared fluorescence scanner used for DNA analysis", Proc. SPIE 2388, Advances in Fluorescence Sensing Technology II, (8 May 1995); doi: 10.1117/12.208522; https://doi.org/10.1117/12.208522
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