Fluorescence lifetime spectroscopy and imaging in random media

Christina L. Hutchinson; Tamara L. Troy; Eva Marie Sevick-Muraca

doi:10.1117/12.209977

30 May 1995 Fluorescence lifetime spectroscopy and imaging in random media

Christina L. Hutchinson, Tamara L. Troy, Eva Marie Sevick-Muraca

Proceedings Volume 2389, Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation; (1995) https://doi.org/10.1117/12.209977
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Tissue fluorescence, whether from endogenous or exogenous probes, provides an opportunity for interrogation on the basis of structure and function. However to date, there has been little understanding of the localization of signal origin of fluorescence signals re-emitted from tissues. Previously, we have shown using finite element computations that the origin or re- emitted fluorescence signal depends upon the lifetime of the optical probe. The signal arising from long-lived phosphorescent probes may predominately come from the tissue-air interface rather than from deep within the tissue. Therefore, the use of short-lived optical probes may be desirable for fluorescence spectroscopy and as contrast agents for biomedical optical lived probes embedded within scattering media. Our results show that upon proper referencing of frequency-domain measurements, lifetime measurements can be made in the presence of uniform and nonuniform distribution of optical probes. The implications for biomedical optical imaging on the basis of probe lifetime are discussed.

Citation Download Citation

Christina L. Hutchinson, Tamara L. Troy, and Eva Marie Sevick-Muraca "Fluorescence lifetime spectroscopy and imaging in random media", Proc. SPIE 2389, Optical Tomography, Photon Migration, and Spectroscopy of Tissue and Model Media: Theory, Human Studies, and Instrumentation, (30 May 1995); https://doi.org/10.1117/12.209977

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