Photothermal microscopy for cell imaging and diagnostics

Dmitry Lapotko; Georgy Kuchinsky

doi:10.1117/12.205988

30 March 1995 Photothermal microscopy for cell imaging and diagnostics

Dmitry Lapotko, Georgy Kuchinsky

Proceedings Volume 2390, Optical Biophysics; (1995) https://doi.org/10.1117/12.205988
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Dual-laser photothermal (PT) techniques are applied for a live blood cell studies. The measurement of the action of a pump pulse (400-600 nm, 10 ns, 1-500 pJ) on a single cell provides the imaging of cell's absorbing structure/thermal field, spectroscopy and control of cell's state. The imaging technique includes acquisition of three types of images: optical image, pulsed laser image with 5 ns temporal resolution and PT-image with the same temporal and 0.5 jim spatial resolution. In PT-images of WBC the details of a cell structure have been found that can not be obtained from optical images of these cells. Quantitative analysis of PT-images enables abnormal cell differentiation in a case of anaemia (RBC) and pulinanaiy diseases (neutrophyls). Analysis of cell population according to the cell ability to survive a pump pulse is considered as the viability test. This technique was used for RBC comparative studie. Significant differences in RBC populations have been found. Also the viability test was applied for monitoiing of action of the drugs upon RBC membrane. Fully automated experimental system was built for PT-studies of the cell populations. All set up is technically compatible to optical microscope. Operation rate is 1 cell/s. The required capacity of cell suspension is about 1 j.d. Keywords: image cytometry, photothermal microscopy.

Citation Download Citation

Dmitry Lapotko and Georgy Kuchinsky "Photothermal microscopy for cell imaging and diagnostics", Proc. SPIE 2390, Optical Biophysics, (30 March 1995); https://doi.org/10.1117/12.205988

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