Does low intensity He-Ne laser radiation affect the intracellular pH of intact Escherichia coli cells?

Terence I. Quickenden; Lillian L. L. Daniels; Lyndsay T. Byrne

doi:10.1117/12.209924

22 May 1995 Does low intensity He-Ne laser radiation affect the intracellular pH of intact Escherichia coli cells?

Terence I. Quickenden, Lillian L. L. Daniels, Lyndsay T. Byrne

Proceedings Volume 2391, Laser-Tissue Interaction VI; (1995) https://doi.org/10.1117/12.209924
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Claims that low levels of He-Ne laser light (cw, (lambda) equals 632.8 nm) can provide clinical benefits and can enhance in vitro cellular growth are still controversial (T.I. Quickenden and L.L. Daniels, 1993, Photochem. Photobiol. 57, 272-278; L.L. Daniels and T.I. Quickenden, 1994, Photochem. Photobiol., 60, 481-485). The present study tests the suggestion (T.I. Karu, 1988, Lasers life Sci. 2, 53-74; H. Friedmann, R. Lubart, I. Laulicht and S. Rochkink, 1991, J. Photochem. Photobiol. B: Biol. 11, 87-91) that red light stimulates mitosis by raising intracellular pH via absorption by chromophores in the respiratory chain. In order to search for photoinduced changes in intracellular pH, the effect of 5 mW He-Ne laser irradiation on cultures of E. coli was examined using a 300 MHz Nuclear Magnetic Resonance (NMR) spectrometer. The pH difference between the intracellular and extracellular fluid was monitored in the presence and absence of radiation by determining the difference in chemical shift for ³¹P resonances arising from the H₂PO₄^- ⇔ HPO₄^2- + H⁺ equilibrium in the two environments.

Citation Download Citation

Terence I. Quickenden, Lillian L. L. Daniels, and Lyndsay T. Byrne "Does low intensity He-Ne laser radiation affect the intracellular pH of intact Escherichia coli cells?", Proc. SPIE 2391, Laser-Tissue Interaction VI, (22 May 1995); https://doi.org/10.1117/12.209924

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