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22 May 1995 Fluorescence imaging and spectroscopy of motile sperm cells and CHO cells in an optical trap (laser tweezers)
Karsten Koenig, Yagang Liu, Tatiana B. Krasieva, Pasquale Patrizio, Yona Tadir M.D., Gregory J. Sonek, Michael W. Berns, Bruce J. Tromberg
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Proceedings Volume 2391, Laser-Tissue Interaction VI; (1995) https://doi.org/10.1117/12.209888
Event: Photonics West '95, 1995, San Jose, CA, United States
Abstract
We describe fluorescence spectroscopy and imaging studies of optically trapped single Chinese hamster ovary (CHO) and motile human sperm cells. The NIR trapping beam was provided by a tunable, multimode continuous wave Ti:Sapphire laser. The beam was introduced into an inverted confocal laser scanning microscope. Fluorescence of cells in the single- beam gradient force optical trap was excited with a 488 nm microbeam (laser scanning microscopy) or with 365 nm radiation from a high- pressure mercury lamp. Modifications to NADH-attributed autofluorescence and Rhodamine- and Propidium Iodide-attributed xenofluorescence indicate a significant cell-damaging effect of 760 nm trapping beams. 760 nm effects produce a biological response comparable to UVA-induced oxidative stress and appear to be a consequence to two-photon absorption.
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Karsten Koenig, Yagang Liu, Tatiana B. Krasieva, Pasquale Patrizio, Yona Tadir M.D., Gregory J. Sonek, Michael W. Berns, and Bruce J. Tromberg "Fluorescence imaging and spectroscopy of motile sperm cells and CHO cells in an optical trap (laser tweezers)", Proc. SPIE 2391, Laser-Tissue Interaction VI, (22 May 1995); https://doi.org/10.1117/12.209888
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KEYWORDS
Luminescence
Imaging spectroscopy
Optical tweezers
Fluorescence spectroscopy
Near infrared
Laser scanners
Spectroscopy
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