Quantitative fluorescence microscopy yields distributions of concentration and photobleaching rate in 5ALA-induced protoporphyrin IX in cell population

Kunio Awazu; Steven L. Jacques

doi:10.1117/12.208176

1 May 1995 Quantitative fluorescence microscopy yields distributions of concentration and photobleaching rate in 5ALA-induced Protoporphyrin IX in cell population

Kunio Awazu, Steven L. Jacques

Author Affiliations +

Proceedings Volume 2392, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IV; (1995) https://doi.org/10.1117/12.208176
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

The variation in photodynamic dose achieved with protoporphyrin Ix (PPIX) and laser radiation was assessed in a cancer cell culture. The distributions of initial PPIX concentration, photobleaching light dose, and photodynamic dose were measured on 124 individual cells (rat mammary cancer cell line, MTF7) using video fluorescence microscopy.

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Kunio Awazu and Steven L. Jacques "Quantitative fluorescence microscopy yields distributions of concentration and photobleaching rate in 5ALA-induced Protoporphyrin IX in cell population", Proc. SPIE 2392, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy IV, (1 May 1995); https://doi.org/10.1117/12.208176

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