Diagnostic fluorescence spectroscopy of oral mucosa

Krishnendu Roy; Ian Bottrill M.D.; Duncan R. Ingrams; Michail M. Pankratov; Elie E. Rebeiz M.D.; Peak Woo; Sadru Kabani; Stanley M. Shapshay M.D.; Ramasamy Manoharan; Irving Itzkan; Michael S. Feld

doi:10.1117/12.209094

12 May 1995 Diagnostic fluorescence spectroscopy of oral mucosa

Krishnendu Roy, Ian Bottrill M.D., Duncan R. Ingrams, Michail M. Pankratov, Elie E. Rebeiz M.D., Peak Woo, Sadru Kabani, Stanley M. Shapshay M.D., Ramasamy Manoharan, Irving Itzkan, Michael S. Feld

Author Affiliations +

Proceedings Volume 2395, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V; (1995) https://doi.org/10.1117/12.209094
Event: Photonics West '95, 1995, San Jose, CA, United States

Abstract

Autofluorescence characteristics of normal, dysplastic, and malignant squamous tissues from the oral cavity were measured with a spectrofluorometer in the excitation range of 250 - 500 nm and emission range of 350 - 750 nm. Fluorescence excitation-emission matrices (EEM) were obtained from samples collected from patients in the clinic and in the operating room. The same samples were submitted for histopathological examination following spectroscopic measurements. The contour plots obtained from the EEMs of the samples showed consistent differences between normal and abnormal tissues. All the abnormal samples showed enhanced red region (> 600 nm) fluorescence with a prominent peak at 635 nm, when excited by 410 nm light. A ratio contour plot (abnormal/normal) enhanced spectral differences in the red region. A fiber-optic based spectrofluorometer for EEM measurements is being developed for further investigations.

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Krishnendu Roy, Ian Bottrill M.D., Duncan R. Ingrams, Michail M. Pankratov, Elie E. Rebeiz M.D., Peak Woo, Sadru Kabani, Stanley M. Shapshay M.D., Ramasamy Manoharan, Irving Itzkan, and Michael S. Feld "Diagnostic fluorescence spectroscopy of oral mucosa", Proc. SPIE 2395, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems V, (12 May 1995); https://doi.org/10.1117/12.209094

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