Monitoring of microbial communities by flow cytometry and rRNA-targeted hybridization probes

Guenter Wallner; Rudolf Amann

doi:10.1117/12.224148

9 October 1995 Monitoring of microbial communities by flow cytometry and rRNA-targeted hybridization probes

Guenter Wallner, Rudolf Amann

Proceedings Volume 2504, Environmental Monitoring and Hazardous Waste Site Remediation; (1995) https://doi.org/10.1117/12.224148
Event: European Symposium on Optics for Environmental and Public Safety, 1995, Munich, Germany

Abstract

Flow cytometry in combination with ribosomal RNA (rRNA) based fluorescence in situ hybridization is a new technique for the analysis of microbial communities. Oligonucleotide probes directed against ribosomal RNA allow the identification of species or groups of micro- organisms. Combined with flow cytometry, up to several thousand cells per second can be classified. In addition to the identification and specific enumeration of micro-organisms, further information on the distribution of cell size, DNA and ribosome content -- and therefore an assessment of activity -- within the entire community or subpopulations can be obtained. This technique is much more accurate, informative, and rapid than classical culture-dependent methods. Data of activated sludge samples hybridized with fluorescein labeled oligonucleotides and counterstained with the DNA-specific dye Hoechst 33342 are presented as examples for its applicability to complex microbial communities.

Citation Download Citation

Guenter Wallner and Rudolf Amann "Monitoring of microbial communities by flow cytometry and rRNA-targeted hybridization probes", Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); https://doi.org/10.1117/12.224148

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