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9 October 1995 Monitoring of microbial communities by flow cytometry and rRNA-targeted hybridization probes
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Proceedings Volume 2504, Environmental Monitoring and Hazardous Waste Site Remediation; (1995) https://doi.org/10.1117/12.224148
Event: European Symposium on Optics for Environmental and Public Safety, 1995, Munich, Germany
Abstract
Flow cytometry in combination with ribosomal RNA (rRNA) based fluorescence in situ hybridization is a new technique for the analysis of microbial communities. Oligonucleotide probes directed against ribosomal RNA allow the identification of species or groups of micro- organisms. Combined with flow cytometry, up to several thousand cells per second can be classified. In addition to the identification and specific enumeration of micro-organisms, further information on the distribution of cell size, DNA and ribosome content -- and therefore an assessment of activity -- within the entire community or subpopulations can be obtained. This technique is much more accurate, informative, and rapid than classical culture-dependent methods. Data of activated sludge samples hybridized with fluorescein labeled oligonucleotides and counterstained with the DNA-specific dye Hoechst 33342 are presented as examples for its applicability to complex microbial communities.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Guenter Wallner and Rudolf Amann "Monitoring of microbial communities by flow cytometry and rRNA-targeted hybridization probes", Proc. SPIE 2504, Environmental Monitoring and Hazardous Waste Site Remediation, (9 October 1995); https://doi.org/10.1117/12.224148
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