6 September 1995 Near-field scanning optical microscope in biological research
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Some problems in a near-field scanning optical microscope (NSOM) system for observing biological living specimens is discussed. The NSOM system works based on the principle of nano-optics and near-field optics. Propagating characteristics of evanescent wave field, the effects of absorption, polarization changes, fluorescence, optical density, refractive index, luminescence and incident angle are analyzed. Methods eliminating pseudo-image in specimens are proposed. NSOM will be employed to conduct investigation of single molecule of living biological specimen and make precision metrology researches in nanometer scale.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jia Wang, Jia Wang, Dacheng Li, Dacheng Li, } "Near-field scanning optical microscope in biological research", Proc. SPIE 2535, Near-Field Optics, (6 September 1995); doi: 10.1117/12.218688; https://doi.org/10.1117/12.218688

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