Confocal scanning microscopy under ultrashort pulse illumination

Min Gu; T. Tannous; Colin J. R. Sheppard

doi:10.1117/12.218311

1 September 1995 Confocal scanning microscopy under ultrashort pulse illumination

Min Gu, T. Tannous, Colin J. R. Sheppard

Proceedings Volume 2549, Ultrahigh- and High-Speed Photography, Videography, and Photonics '95; (1995) https://doi.org/10.1117/12.218311
Event: SPIE's 1995 International Symposium on Optical Science, Engineering, and Instrumentation, 1995, San Diego, CA, United States

Abstract

We, in this paper, investigate resolution in various confocal imaging methods based on the use of an ultrashort pulsed laser beam. For bright-field confocal microscopy, time-resolved and time averaged imaging methods are considered, while resolution in two-photon and single- photon confocal fluorescence microscopy is examined. It is shown that the time-resolved approach can give a 3D image of a thick object with axial resolution approximately 30% higher than the limiting value under CW laser illumination of the same wavelength as the pulsed beam, while the transverse resolution is slightly improved.

Citation Download Citation

Min Gu, T. Tannous, and Colin J. R. Sheppard "Confocal scanning microscopy under ultrashort pulse illumination", Proc. SPIE 2549, Ultrahigh- and High-Speed Photography, Videography, and Photonics '95, (1 September 1995); https://doi.org/10.1117/12.218311

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