24 November 1995 Laser remote monitoring of plant photosynthetic activity
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Abstract
Laboratory measurements of laser induced chlorophyll fluorescence kinetics (Kautsky effect) on dark-adapted vegetation targets (maize, pine-tree) have been performed with a lidar fluorosensor by superimposing probe pulses upon an actinic light. The collected induction curves (fast rise and slow decline) have been used to reveal the occurrence of stresses and the damage produced by a pine-tree parasite. A new two-pulse LIF (laser induced fluorescence) methodology has been investigated both theoretically and experimentally, in view of remotely monitoring the plant photosynthetic activity. This technique may yield information upon the in-vivo photosynthetic processes of plants, revealing a possible stress status (nutrients depletion, presence of herbicides, photoinhibition, etc.). The lidar apparatus used contains two laser sources in order to differentially measure the chlorophyll fluorescence by means of a laser pump-and-probe technique. In fact LIF signals in the red chlorophyll band 690 nm may provide in-vivo information upon photosynthesis process in high order plants and algae. Laser pump-and-probe experimental tests, with excitation 355 nm or 532 nm, already detect the presence of herbicides, and the effects of plant exposure to thermal stresses and to low levels of gaseous pollutants. Laser measured fluorescence yields (Y) have been found to be consistent with those obtained by an in-situ fluorimeter (PAM). With proper choices of experimental parameters (pump and probe laser intensities), Y approaches the theoretical value expected for a healthy dark-adapted plant.
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Roberto Barbini, Roberto Barbini, Francesco Colao, Francesco Colao, Roberta Fantoni, Roberta Fantoni, Antonio Palucci, Antonio Palucci, Sergio Ribezzo, Sergio Ribezzo, "Laser remote monitoring of plant photosynthetic activity", Proc. SPIE 2585, Remote Sensing for Agriculture, Forestry, and Natural Resources, (24 November 1995); doi: 10.1117/12.227169; https://doi.org/10.1117/12.227169
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