19 January 1996 Biocompatibility of Er:YSGG laser radiated root surfaces
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Abstract
Pulsed Er:YAG and Er:YSGG lasers are well known to be effective instruments for the ablation of dental hard tissues. Developments in the last years made it possible to transmit the laser radiation at these wavelengths with flexible fibers. Therefore the application in the periodontal pocket may be possible. The aim of this study was to evaluate the in-vitro conditions to generate a bioacceptable root surface. Twenty extracted human teeth, stored in an antibiotic solution, were conventionally scaled, root planed and axially separated into two halves. Two main groups were determined. With the first group laser radiation was carried out without and in the second group with spray cooling. The laser beam was scanned about root surface areas. Laser parameters were varied in a selected range. The biocompatibility was measured with the attachment of human gingival fibroblasts and directly compared to conventionally treated areas of the root surfaces. The fibroblasts were qualified and counted in SEM investigations. On conventionally treated areas gingival fibroblasts show the typical uniform cover. In dependance on the root roughness after laser treatment the fibroblasts loose the typical parallel alignment to the root surface. With spray cooling a better in-vitro attachment could be obtained. Without spray cooling the higher increase in temperature conducted to less bioacceptance by the human gingival fibroblasts to the root surface. These results show the possibility of producing bioacceptable root surfaces with pulsed laser radiation in the range of very high water absorption near 3 micrometer.
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Hartmut Benthin, Hartmut Benthin, Thomas P. Ertl, Thomas P. Ertl, Dirk Schmidt, Dirk Schmidt, Peter Purucker, Peter Purucker, J.-P. Bernimoulin, J.-P. Bernimoulin, Gerhard J. Mueller, Gerhard J. Mueller, } "Biocompatibility of Er:YSGG laser radiated root surfaces", Proc. SPIE 2623, Medical Applications of Lasers III, (19 January 1996); doi: 10.1117/12.230328; https://doi.org/10.1117/12.230328
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