Identification of spectroscopic and optical parameters of whole blood depending on its concentration and layer thickness

Alexandre Yu. Douplik; Victor B. Loschenov

doi:10.1117/12.229550

10 January 1996 Identification of spectroscopic and optical parameters of whole blood depending on its concentration and layer thickness

Alexandre Yu. Douplik, Victor B. Loschenov

Author Affiliations +

Proceedings Volume 2624, Laser-Tissue Interaction and Tissue Optics; (1996) https://doi.org/10.1117/12.229550
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

Optical parameters such as total and diaphragm transmission and diffuse reflection have been investigated by in vitro set up for whole blood. Original construction cuvette allows change thickness of a layer inside without removing of content. Changes of the blood concentration have been created by dilution of centrifuged blood. Hematocrit, hemoglobin and oxyhemoglobin concentration have been measured simultaneously by independent methods. According to the received data the coefficients of scattering and absorption and the average cosine of scattering are calculated for whole blood by the Monte-Carlo simulations. The curve of light penetration into whole blood is created for the following wavelengths: 488, 587, 633, 675, 820, 860 nm.

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Alexandre Yu. Douplik and Victor B. Loschenov "Identification of spectroscopic and optical parameters of whole blood depending on its concentration and layer thickness", Proc. SPIE 2624, Laser-Tissue Interaction and Tissue Optics, (10 January 1996); https://doi.org/10.1117/12.229550

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