31 January 1996 Comparison of two phthalocyanines for PDT
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Abstract
Photodynamic therapy (PDT) is a new treatment modality for bladder carcinoma, especially carcinoma in situ. The goal of our study was to compare two different phthalocyanines for PDT of bladder carcinoma cells and to evaluate phototoxic potential. Material and methods: For PDT hydrophilic chlor-aluminum sulfonated phthalocyanine (CASP) and lipophilic zinc- phthalocyanine (ZnP, CPG 55 847) were compared. For ZnP liposomes as drug carrier were used. The photosensitizer concentrations for both substances used for PDT experiments were 5, 10 and 20 (mu) g/ml. Irradiation was performed with a Penta lamp emitting at wavelengths between 590 and 900 nm at a fluence of up to 12 J/cm2. After irradiation cells were incubated for two days, counted and compared with a control group. Results: The cell survival rate was decreased depending on the light and drug dose. After incubation with the highest drug dose and irradiation with 12 J/cm2 cell survival was 0.03 and 5.5% after incubation with CASP or ZnP, respectively. Light, CASP or ZnP alone had no effect on cells. Studies of the PDT effect by electron microscopy showed intracellular vacuolization caused by mitochondrial damage after incubation with either photosensitizer. Conclusion: The hydrophilic and lipophilic phthalocyanine tested here showed very similar effects on our cell line. ZnP is a pure compound and hence has some advantage over CASP, which is a mixture of a tri- and tetrasulfonated phthalocyanine. In addition, ZnP is administered in liposomes which should enhance tumor selectivity by binding to low density lipoprotein receptors on tumor cells.
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Ruediger Bachor, Ruediger Bachor, Ella Dumbravia Reich, Ella Dumbravia Reich, Peter Graf, Peter Graf, Angelika C. Rueck, Angelika C. Rueck, Richard E. Hautmann, Richard E. Hautmann, } "Comparison of two phthalocyanines for PDT", Proc. SPIE 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities, (31 January 1996); doi: 10.1117/12.230979; https://doi.org/10.1117/12.230979
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