Phototoxicity, dark-toxicity, and uptake-kinetics of natural hydrophilic and hydrophobic porphyrins in endothelial cells

Nermin Akguen; Reinhard Sailer; Karin Kunzi-Rapp; Herbert Schneckenburger; Gerd C. Beck; Angelika C. Rueck

doi:10.1117/12.231003

31 January 1996 Phototoxicity, dark-toxicity, and uptake-kinetics of natural hydrophilic and hydrophobic porphyrins in endothelial cells

Nermin Akguen, Reinhard Sailer, Karin Kunzi-Rapp, Herbert Schneckenburger, Gerd C. Beck, Angelika C. Rueck

Author Affiliations +

Proceedings Volume 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities; (1996) https://doi.org/10.1117/12.231003
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

The phototoxicity, darktoxicity and uptake kinetics of three natural porphyrins (Uropoprhyrin III; UP III, Coproporphyrin III; CP III and Protoporphyrin IX; PP IX) were investigated in vitro using the BKEz-7 aorta endothelial cells of the calf. The cells were incubated with the porphyrins in different concentrations (0.5 (mu) M PP IX; 50 (mu) M UP III and CP III). After 24 h incubation they were irradiated in the case of PP IX with an Ar⁺-dye- laser at 635 nm and in the case of UP III and CP III with a Kr⁺-laser at 407 nm: While PP IX was phototoxic at low concentrations (0.5 (mu) M) and low energies (10 J/cm²), irradiation of UP III and CP III hardly induced phototoxicity even at higher concentrations. The same could be observed for the darktoxicity. PP IX was darktoxic at relatively low concentrations (1 (mu) M). In addition PP IX was taken up much faster and in greater amounts into the endothelial cells than UP III and CP III. These results could be due to the different structures of the sensitizers and/or to different uptake mechanisms. PP IX is a hydrophobic sensitizer while UP III and CP III are both hydrophilic molecules. A different uptake mechanism and accumulation in endothelial cells is quite probable. This hypothesis was confirmed with video-microscopy. In addition to the experiments in vitro, the cellular uptake and distribution of the sensitizers were observed in an appropriate in vivo model of the Chorioallantoismembrane (CAM).

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Nermin Akguen, Reinhard Sailer, Karin Kunzi-Rapp, Herbert Schneckenburger, Gerd C. Beck, and Angelika C. Rueck "Phototoxicity, dark-toxicity, and uptake-kinetics of natural hydrophilic and hydrophobic porphyrins in endothelial cells", Proc. SPIE 2625, Photochemotherapy: Photodynamic Therapy and Other Modalities, (31 January 1996); https://doi.org/10.1117/12.231003

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KEYWORDS

Photodynamic therapy

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In vitro testing

Luminescence

Content addressable memory

Cameras

Fluorescence correlation spectroscopy

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