18 December 1995 Frequency domain technique for a two-dimensional mapping of optical tissue properties
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Locally and individually varying optical tissue parameters (mu) a, (mu) s, and g are responsible for non-neglectible uncertainties in the interpretation of spectroscopic data in optical biopsy techniques. The intrinsic fluorescence signal for instance doesn't depend only on the fluorophore concentration but also on the amount of other background absorbers and on alterations of scattering properties. Therefore neither a correct relative nor an absolute mapping of the lateral fluorophore concentration can be derived from the intrinsic fluorescence signal alone. Using MC-simulations it can be shown that in time-resolved LIFS the simultaneously measured backscattered signal of the excitation wavelength (UV) can be used to develop a special, linearized rescaling algorithm to take into account the most dominant of these varying tissue parameters which is (mu) a,ex. In combination with biochemical calibration measurements we were able to perform fiberbased quantitative NADH- concentration measurements. In this paper a new rescaling method for VIS and IR light in the frequency domain is proposed. It can be applied within the validity range of the diffusion approximation and provides full (mu) a and (mu) s rescaling possibility in a 2- dimensional, non-contact mapping mode. The scanning device is planned to be used in combination with a standard operation microscope of ZEISS, Germany.
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Thomas Bocher, Thomas Bocher, Juergen Beuthan, Juergen Beuthan, Olaf Minet, Olaf Minet, Rolf-Dieter Naber, Rolf-Dieter Naber, Gerhard J. Mueller, Gerhard J. Mueller, } "Frequency domain technique for a two-dimensional mapping of optical tissue properties", Proc. SPIE 2626, Photon Propagation in Tissues, (18 December 1995); doi: 10.1117/12.228676; https://doi.org/10.1117/12.228676

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