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15 January 1996 Image enhancement of microscopic fluorescence images
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Two methods are proposed to improve microscopic fluorescence images. In the first method, the second derivative of the image is taken with respect to the focal depth. To obtain the derivative, the focus of the microscope objective is modulated piezoelectrically by 5 - 10 micrometer, and three images with focal positions z plus h, z and z minus h are taken. The resulting image is calculated according to the formula h2 f'(z) approximately equals 2f(z)-f(z plus h)- f(z minus h) that follows immediately from Taylors formula. In the second method, a 2D- Fourier transform is applied to the image, followed by multiplication with a model function that reduces low spatial frequencies. Then, the image is transformed back into the spatial domain. The improvements are demonstrated on fluorescence microscopical images of bronchial tumor tissue.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Volkmar Betz and Joerg-Uwe Meyer "Image enhancement of microscopic fluorescence images", Proc. SPIE 2628, Optical and Imaging Techniques for Biomonitoring, (15 January 1996);

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