8 January 1996 Cell patterns for biosensors manufactured with laser tweezers
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Abstract
A drug screening assay based on patterned cells was developed. The patterning was performed by detaching single lymphocytes with laser tweezers from a poly-ethylene oxide (PEO) surface and immobilizing them on a surface coated with Cell-TakR, a strong cellular adhesive. The detachment force of the cells from the PEO-surface was determined on a single cell base with the laser tweezers to be between 1.5 and 4.5 pN. Different lymphocyte classes were loaded with the fluorescent calcium indicator Fluo-3 and the pH indicator BCECF. Specific stimulation of the immobilized cells is monitored.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Joachim Renken, Joachim Renken, Stefan Seeger, Stefan Seeger, } "Cell patterns for biosensors manufactured with laser tweezers", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); doi: 10.1117/12.229505; https://doi.org/10.1117/12.229505
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