Ultrasensitive detection of cholecystokinin (CCK) by laser-induced time-resolved fluorescence diagnostics

Rene Paul; Lutz Pfeifer; Klaus Kemnitz; Frank Fink

doi:10.1117/12.229512

8 January 1996 Ultrasensitive detection of cholecystokinin (CCK) by laser-induced time-resolved fluorescence diagnostics

Rene Paul, Lutz Pfeifer, Klaus Kemnitz, Frank Fink

Author Affiliations +

Proceedings Volume 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology; (1996) https://doi.org/10.1117/12.229512
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

An ultrasensitive detection method of CCK in aqueous solution was developed. This method utilizes intrinsic native fluorescence properties of CCK-molecules. A new delay-line multichannel plate photomultiplier (MCP PMT) was used to measure both time and wavelength resolved fluorescence properties simultaneously. Both CCK4 and CCK8 showed biexponential fluorescence decay. While the short lived fluorescence decay time components were rather similar, the long-lived components differed by about 1 ns. In the case of CCK8 the long-lived component was red-shifted by 30 nm. Based on these photophysical data an experimental setup for an ultrasensitive detection was developed. The lowest detection limit was achieved by investigating the fluorescence intensity within a time gate (gate duration 2 ns) in the fluorescence maximum. In combination with a confocal setup, using a Cassegrainean configuration, CCK could be detected up to 10^-12 M.

Citation Download Citation

Rene Paul, Lutz Pfeifer, Klaus Kemnitz, and Frank Fink "Ultrasensitive detection of cholecystokinin (CCK) by laser-induced time-resolved fluorescence diagnostics", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); https://doi.org/10.1117/12.229512

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