8 January 1996 Using luminescent ruthenuim(II) complexes as dyes in the multiplex concept
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The detection of several substances in a mixture can be realized by using luminescent markers and discriminating them by their luminescence lifetimes. The number of simultaneously detectable analytes can be increased by extending the time region of the lifetimes of the dyes. The fluorescence lifetimes of commercial available organic dyes do not exceed 100 ns. Longer decays are found among the complexes of transition metals. Therefore the photophysical properties of a number of luminescent ruthenium(II) tris(alpha-diimine) complexes in air equilibrated aqueous buffer solution were measured at room temperature. Their application as luminescence markers in diagnostics and analytics is discussed. The possibility of emission enhancement by addition of detergents to reduce water quenching and by addition of sodiumsulfite to reduce oxygen quenching was investigated. Some of the ruthenium compounds were encapsulated in carboxylated polystyrol beads. Resulting changes of the luminescence decays are presented. The possibility of increasing sensitivity by multilabeling with dyed beads in analytical applications is discussed.
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Brigitte Ullrich, Brigitte Ullrich, Stefan Seeger, Stefan Seeger, Juergen M. Wolfrum, Juergen M. Wolfrum, } "Using luminescent ruthenuim(II) complexes as dyes in the multiplex concept", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); doi: 10.1117/12.229516; https://doi.org/10.1117/12.229516

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