15 January 1996 Effects of the 633-nm laser on the behavior and morphology of primary fibroblast culture
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Proceedings Volume 2630, Effects of Low-Power Light on Biological Systems; (1996); doi: 10.1117/12.230027
Event: BiOS Europe '95, 1995, Barcelona, Spain
Abstract
We previously described the influence of low-level laser therapy (LLLT) on the primary fibroblast ATCC CRL1471 CCD-19SK passage 7 in culture, metabolic changes and statistical significance absorption of 3[H]Hydroxyproline after 2 irradiations (12 hour intervals) with Ar:DYE Laser, 633 nm wavelength, output power 38 mw, spot size 3.5 cm, power density 4 mw/cm2, energy density plus or minus 2 J/cm2. The aim of this work is to investigate, by using the same procedure, the behavior of the confluence monolayer fibroblasts culture when a central scratch of 0.4 - 1 mm and 2 irradiations is performed, by means of the study of the colony formation, haptotaxis (direction) and chemotaxis- chemokinesis (movement). The results indicate that all these phenomena appear sooner in the LLLT cultures than in non-treated cultures. Thus we can confirm that LLLT induces fibroblast biological effects.
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Josepa Rigau, Chung-Ho Sun, Mario A. Trelles, Michael W. Berns, "Effects of the 633-nm laser on the behavior and morphology of primary fibroblast culture", Proc. SPIE 2630, Effects of Low-Power Light on Biological Systems, (15 January 1996); doi: 10.1117/12.230027; https://doi.org/10.1117/12.230027
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KEYWORDS
Laser therapeutics

In vitro testing

Wound healing

Absorption

Microscopy

CCD cameras

Helium neon lasers

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