Biochemical affinity sensing systems based on luminescence generation in the evanescent field of optical waveguides

Gert L. Duveneck; Peter Oroszlan; A. P. Abel; B. Klee; V. Steiner; Markus Ehrat; D. Gygax; H. Michael Widmer

doi:10.1117/12.229177

3 January 1995 Biochemical affinity sensing systems based on luminescence generation in the evanescent field of optical waveguides

Gert L. Duveneck, Peter Oroszlan, A. P. Abel, B. Klee, V. Steiner, Markus Ehrat, D. Gygax, H. Michael Widmer

Author Affiliations +

Proceedings Volume 2631, Medical and Fiber Optic Sensors and Delivery Systems; (1995) https://doi.org/10.1117/12.229177
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

We have developed a (bio)chemical analysis system based on luminescence generation and detection in the evanescent field associated with light guiding in an optical fiber. Our intention was directed towards optimization of not only the sensor, including the sensor handling and the immobilization of biochemical recognition elements, but also of the assay chemistry, with special emphasis on methods used for sensor regeneration, of the fluidic system, and of the experimental control software. Goals of this optimization process were not only to achieve high sensitivity, reproducibility and the related precision of the results, but also maximum regenerability of the sensors and system flexibility for a variety of different applications. Four examples of different bioaffinity assays, established on our sensor system, are presented: a competitive immunoassay for atrazine, a sandwich immunoassay for hirudin, a DNA hybridization assay, and first studies for the development of sensors based on membrane- bound receptors. In the atrazine assay, the sensor could be regenerated more than 300 times. In the hybridization assay, a detection limit of 7.5 multiplied by 10^-14 M complementary fluorescein-labeled DNA was achieved. The performance of our system is compared with an established enzyme-linked immunosorbent assay (ELISA) on the example of the hirudin assay. In the concluding section of this paper, advantages and disadvantages of our fiberoptic, luminescence-based system, compared with commercialized systems, based on detection of changes of the effective refractive index, are discussed.

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Gert L. Duveneck, Peter Oroszlan, A. P. Abel, B. Klee, V. Steiner, Markus Ehrat, D. Gygax, and H. Michael Widmer "Biochemical affinity sensing systems based on luminescence generation in the evanescent field of optical waveguides", Proc. SPIE 2631, Medical and Fiber Optic Sensors and Delivery Systems, (3 January 1995); https://doi.org/10.1117/12.229177

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