10 April 1996 Fluorescence microtomography: multiangle image acquisition and 3D digital reconstruction
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We have developed a prototype fluorescence microscope which, using tomographic image acquisition and reconstruction techniques, can automatically combine conventional and/or confocal image stacks taken at a number of orientations into a single, very-high-resolution 3D image. We use the term `microtomography' in a broad sense to denote digital image reconstruction from multiple imaging operations which are not necessarily projections. Our system holds a biological specimen inside a thin capillary tube which is rotatable over a 360 degree range beneath an immersion objective. 3D fluorescence image data volumes are acquired by frame-grabbing a through-focus series of 2D images at each angle of rotation. Digital reconstruction of the multi-angle data volumes produces a single very-high-resolution 3D image and involves algorithms which perform rotation, interpolation, alignment and normalization operations in frequency (Fourier) space.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Carol J. Cogswell, Carol J. Cogswell, Kieran G. Larkin, Kieran G. Larkin, Hanno U. Klemm, Hanno U. Klemm, } "Fluorescence microtomography: multiangle image acquisition and 3D digital reconstruction", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237467; https://doi.org/10.1117/12.237467

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