10 April 1996 Fluorescence microtomography: multiangle image acquisition and 3D digital reconstruction
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Abstract
We have developed a prototype fluorescence microscope which, using tomographic image acquisition and reconstruction techniques, can automatically combine conventional and/or confocal image stacks taken at a number of orientations into a single, very-high-resolution 3D image. We use the term `microtomography' in a broad sense to denote digital image reconstruction from multiple imaging operations which are not necessarily projections. Our system holds a biological specimen inside a thin capillary tube which is rotatable over a 360 degree range beneath an immersion objective. 3D fluorescence image data volumes are acquired by frame-grabbing a through-focus series of 2D images at each angle of rotation. Digital reconstruction of the multi-angle data volumes produces a single very-high-resolution 3D image and involves algorithms which perform rotation, interpolation, alignment and normalization operations in frequency (Fourier) space.
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Carol J. Cogswell, Kieran G. Larkin, Hanno U. Klemm, "Fluorescence microtomography: multiangle image acquisition and 3D digital reconstruction", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237467; https://doi.org/10.1117/12.237467
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