New approach toward improved fluorescence imaging beyond confocal resolution

G. J. Brakenhoff; Michiel Mueller

doi:10.1117/12.237485

10 April 1996 New approach toward improved fluorescence imaging beyond confocal resolution

G. J. Brakenhoff, Michiel Mueller

Proceedings Volume 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III; (1996) https://doi.org/10.1117/12.237485
Event: Electronic Imaging: Science and Technology, 1996, San Jose, CA, United States

Abstract

Based on the principle of spatial autocorrelation of the focal field, improved resolution can be obtained over that obtainable in confocal microscopy. The method is presented and the imaging resolution is analyzed for two model objects. The method employs only one high NA lens for the, potentially robust, common path delivery of the autocorrelated excitation beams.

Citation Download Citation

G. J. Brakenhoff and Michiel Mueller "New approach toward improved fluorescence imaging beyond confocal resolution", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); https://doi.org/10.1117/12.237485

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