10 April 1996 Three-dimensional computed tomography using a double-axis fluorescence microscope
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We developed a double-axis fluorescence microscope as a new type of 3D microscope, in which two conventional microscopes are placed perpendicularly to one another. This optical microscope provides a solution to extend the spatial frequency band in the depth direction, which is greatly limited for conventional single-axis microscopes. In addition, this equipment has a capability of implementing a fast image acquisition system by simultaneously taking images through both microscopes. We evaluated the 3D imaging properties of our system using 3D observation images of a micro-fluorescent sphere. In order to reconstruct 3D images of micro-objects, we applied a CT-based method we proposed to the 3D microscopic imaging system. Our experiments using a biological micro-specimen verified usefulness and effectiveness of our system.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Susumu Kikuchi, Susumu Kikuchi, Kazuo Sonobe, Kazuo Sonobe, Hidetoshi Yamada, Hidetoshi Yamada, Nagaaki Ohyama, Nagaaki Ohyama, } "Three-dimensional computed tomography using a double-axis fluorescence microscope", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237468; https://doi.org/10.1117/12.237468

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