Two- and three-dimensional segmentation and measurement of particles in the analysis of microscopic digital images of biological samples

Billy W. Loo Jr.; Bahram Parvin; Stephen S. Rothman

doi:10.1117/12.237478

10 April 1996 Two- and three-dimensional segmentation and measurement of particles in the analysis of microscopic digital images of biological samples

Billy W. Loo Jr., Bahram Parvin, Stephen S. Rothman

Author Affiliations +

Proceedings Volume 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III; (1996) https://doi.org/10.1117/12.237478
Event: Electronic Imaging: Science and Technology, 1996, San Jose, CA, United States

Abstract

The authors present a Hough transform based image segmentation algorithm for automated detection, counting, and measurement of particles in two- and three-dimensional microscopic digital image data sets. The algorithm has proven to be both sensitive and specific for the particles of interest, even in the presence of noise and blurring. We apply the algorithm to the problem of automated compilation of population statistics on the size and mass of zymogen granules in pancreatic acinar cells. We present results describing the performance of the algorithm on digital phantoms, and image data from conventional fluorescence and confocal microscopes.

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Billy W. Loo Jr., Bahram Parvin, and Stephen S. Rothman "Two- and three-dimensional segmentation and measurement of particles in the analysis of microscopic digital images of biological samples", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); https://doi.org/10.1117/12.237478

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