10 April 1996 Volumetric Raman spectral imaging with a confocal Raman microscope: image modalities and applications
Author Affiliations +
Abstract
The Fourier transform Confocal Raman Microscope (FT-CRM) enables non-invasive 3D Raman spectroscopic analysis and visualization of chemically heterogeneous preparations. The instrument combines a confocal optical microscope with a visible light Fourier transform Raman spectrometer to acquire and analyze the Raman spectrum of light scattered from a voxel in the sample defined by the confocal optics. Scanning the sample relative to the confocal voxel and recording the Raman spectrum at each scan position generates a multi- dimensional data set encoding the spatially-varying compositional properties of the sample. We report here on the spatial and spectral FT-CRM image properties that includes recent work on correlation-based Raman spectroscopic imaging and application of parametric spectral estimators for robust Raman spectrum estimation.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Colin J. H. Brenan, Ian Warwick Hunter, Michael J. Korenberg, "Volumetric Raman spectral imaging with a confocal Raman microscope: image modalities and applications", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); doi: 10.1117/12.237470; https://doi.org/10.1117/12.237470
PROCEEDINGS
10 PAGES


SHARE
Back to Top