23 April 1996 Effect of Nd:YAG collagen synthesis by human pulp fibroblasts
Author Affiliations +
Abstract
The effect of Nd:YAG laser on tissue is dependant on treatment power, times and frequencies. In order to determine the biologic effect Nd:YAG laser radiation on dental pulp tissue, human dental pulp fibroblasts were obtained from extracted human teeth, and grown in DME containing 10% FBS and used as passage 6 - 7. Confluent cells were exposed to defocused laser energy (1 W, 10 Hz) using a pulsed (120 microsecond(s) ) fiberoptic delivered (320 micrometers fiber) Nd:YAG for 1 - 5 minutes. The cells were labeled with 10 (mu) Ci/ml of 3H proline for 16 hours. Untreated cultures were used as controls. Collagen synthesis was expressed as DPM X 10-3 mg/protein. The purpose of these measurements was to ascertain whether these radiation levels may induced a reparative response resulting in enhanced collagen synthesis. The results showed that at 1 - 5 minutes there was not significant effect. The tested lasing doses apparently did not cause any injurious effect to the fibroblasts, hence no increase in collagen synthesis.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Rahmat A. Barkhordar, Rahmat A. Barkhordar, Qaiser P. Ghani, Qaiser P. Ghani, Belma A. Enriquez, Belma A. Enriquez, M. Zamir Hussain, M. Zamir Hussain, } "Effect of Nd:YAG collagen synthesis by human pulp fibroblasts", Proc. SPIE 2672, Lasers in Dentistry II, (23 April 1996); doi: 10.1117/12.238751; https://doi.org/10.1117/12.238751
PROCEEDINGS
4 PAGES


SHARE
RELATED CONTENT

Study Of Balloon Laserthermia
Proceedings of SPIE (September 10 1989)
Laser treatment of hypertrophic scars
Proceedings of SPIE (October 22 2001)
Pulsed lasers in dentistry: sense or nonsense?
Proceedings of SPIE (April 30 1991)

Back to Top