Accumulation of hypericin in human gastric tumors

Ivan S. Melnik; Sergiy M. Dets; Tatyana V. Rusina; Nikolay A. Denisov; Evgeniy M. Braun; Vladimir O. Kikot; Vyacheslav A. Chernyj

doi:10.1117/12.237522

2 April 1996 Accumulation of hypericin in human gastric tumors

Ivan S. Melnik, Sergiy M. Dets, Tatyana V. Rusina, Nikolay A. Denisov, Evgeniy M. Braun, Vladimir O. Kikot, Vyacheslav A. Chernyj

Author Affiliations +

Proceedings Volume 2675, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy V; (1996) https://doi.org/10.1117/12.237522
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Hypericin has been studied as a novel natural photosensitizer for PDT. It has been extracted from plants (St.-John's-wort). Oral administration (10% alcohol solution in a dose 2 mg/kg b.w.) was applied for 15 patients with gastric cancers 18 - 48 h before surgery. Normal and cancerous tissue samples were resected and underwent fluorescence analysis 1 - 2 h after resection. Tissue fluorescence was excited by He-Cd (20 mW, 442 nm) and Ar laser beams (100 mW, 488 nm) and registered from 510 to 725 nm. In tissue hypericin has maximum fluorescence peak at 603 nm for both excitation wavelengths. Fluorescence intensity ratio I₆₀₃/I₅₀₃ chosen as a criterion for tissue classification was varied from 1.6 to 3.2 (mean 2.5) for adenocarcinoma under He-Cd excitation whereas Ar laser excitation gave from 2.5 up to 4.2 (mean 3.5). Normal tissue had this ratio from 0.48 to 0.65 (mean 0.55) and from 0.53 to 0.75 (mean 3.5) for He-Cd and Ar laser excitation, respectively. No side effects were observed in patients during 6 month follow-up.

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Ivan S. Melnik, Sergiy M. Dets, Tatyana V. Rusina, Nikolay A. Denisov, Evgeniy M. Braun, Vladimir O. Kikot, and Vyacheslav A. Chernyj "Accumulation of hypericin in human gastric tumors", Proc. SPIE 2675, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy V, (2 April 1996); https://doi.org/10.1117/12.237522

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