Photobleaching of native and exogenous (BPD-MA) fluorescence: an in-vivo tumor-bearing rat model

Thanassis Papaioannou; Sandor G. Vari M.D.; Vani R. Pergadia; Warren S. Grundfest M.D.; Jean-Michel I. Maarek

doi:10.1117/12.237531

2 April 1996 Photobleaching of native and exogenous (BPD-MA) fluorescence: an in-vivo tumor-bearing rat model

Thanassis Papaioannou, Sandor G. Vari M.D., Vani R. Pergadia, Warren S. Grundfest M.D., Jean-Michel I. Maarek

Proceedings Volume 2675, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy V; (1996) https://doi.org/10.1117/12.237531
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

The photobleaching of the native fluorescence, as well as of the BPD-MA, an exogenous sensitizer, was measured in an in-vivo tumor bearing rat model. The photobleaching was evaluated in the time scale of seconds (approximately 0 sec - 30 sec) under continuous wave laser light irradiation at 441.6 nm. Three different sites were irradiated: the primary tumor, the mesenteric I lymph node, and the right medial iliac lymph node, at two excitation levels of 6.5 mW/mm² (n equals 6), and approximately 35 mW/mm² (n equals 6). Most of the fluorescence curves were adequately characterized by a biexponential decay. Increased levels of illumination were found to accelerate the photobleaching. No involvement of statistical significance of the irradiation site on the fluorescence decay was found.

Citation Download Citation

Thanassis Papaioannou, Sandor G. Vari M.D., Vani R. Pergadia, Warren S. Grundfest M.D., and Jean-Michel I. Maarek "Photobleaching of native and exogenous (BPD-MA) fluorescence: an in-vivo tumor-bearing rat model", Proc. SPIE 2675, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy V, (2 April 1996); https://doi.org/10.1117/12.237531

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