24 April 1996 Electronic speckle pattern interferometry: new applications in biophysics
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Electronic speckle pattern interferometry (ESPI) provides full field interferometric mapping of deformations, vibrations and density fluctuations in test objects. The interference fringes can be followed in real-time while phase-stepping techniques provide quantitative information. Interferometric measurement of biological objects by standard holography has so far been limited by fringe decorrelation due to microstructure changes. ESPI is based on video recording which allows us to monitor relatively rapid changes in biological objects. To illustrate the potentials of the technique, the gravitropical response of oat coleoptiles (seedlings) was studied. A coleoptile represents a fast growing, partly translucent biological object which is difficult to record interferometrically. However, growth and bending of the specimen were measured even on the tip of the coleoptile where the microstructure changes very rapidly. We also show how small temperature changes in transparent objects can be measured. In water, the sensitivity to temperature change is 0.7 multiplied by 10-3 K per meter. Due to a small difference in temperature between the bulk liquid and its droplet, it was possible to follow and measure movement of droplets in liquids.
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Astrid Aksnes Dyrseth, Astrid Aksnes Dyrseth, } "Electronic speckle pattern interferometry: new applications in biophysics", Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); doi: 10.1117/12.238811; https://doi.org/10.1117/12.238811

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