Molecular assembly of multilayer enzyme on fiber optic surface: toward the development of chemiluminescence-based fiber optic biosensors

Zhongping Chen; Harry Hong Gao; Jayant Kumar; Kenneth A. Marx; Sukant K. Tripathy; David L. Kaplan

doi:10.1117/12.238794

24 April 1996 Molecular assembly of multilayer enzyme on fiber optic surface: toward the development of chemiluminescence-based fiber optic biosensors

Zhongping Chen, Harry Hong Gao, Jayant Kumar, Kenneth A. Marx, Sukant K. Tripathy, David L. Kaplan

Author Affiliations +

Proceedings Volume 2676, Biomedical Sensing, Imaging, and Tracking Technologies I; (1996) https://doi.org/10.1117/12.238794
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

We report here on a technique to immobilize a multilayer enzyme assembly on an optic fiber surface. A multilayer of an enzyme, alkaline phosphatase, was successfully immobilized on an optical fiber surface. Chemiluminescence, ellipsometry, and surface plasmon resonance were used to characterize the structure and activity of the assembly. A chemiluminescence-based fiber optic biosensor utilizing this immobilization technique has been developed for the detection of organophosphorous-based pesticides. Detection of pesticide at sub-ppm level has been achieved for paraoxon.

Citation Download Citation

Zhongping Chen, Harry Hong Gao, Jayant Kumar, Kenneth A. Marx, Sukant K. Tripathy, and David L. Kaplan "Molecular assembly of multilayer enzyme on fiber optic surface: toward the development of chemiluminescence-based fiber optic biosensors", Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); https://doi.org/10.1117/12.238794

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available