24 April 1996 Molecular assembly of multilayer enzyme on fiber optic surface: toward the development of chemiluminescence-based fiber optic biosensors
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Abstract
We report here on a technique to immobilize a multilayer enzyme assembly on an optic fiber surface. A multilayer of an enzyme, alkaline phosphatase, was successfully immobilized on an optical fiber surface. Chemiluminescence, ellipsometry, and surface plasmon resonance were used to characterize the structure and activity of the assembly. A chemiluminescence-based fiber optic biosensor utilizing this immobilization technique has been developed for the detection of organophosphorous-based pesticides. Detection of pesticide at sub-ppm level has been achieved for paraoxon.
© (1996) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Zhongping Chen, Zhongping Chen, Harry Hong Gao, Harry Hong Gao, Jayant Kumar, Jayant Kumar, Kenneth A. Marx, Kenneth A. Marx, Sukant K. Tripathy, Sukant K. Tripathy, David L. Kaplan, David L. Kaplan, } "Molecular assembly of multilayer enzyme on fiber optic surface: toward the development of chemiluminescence-based fiber optic biosensors", Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); doi: 10.1117/12.238794; https://doi.org/10.1117/12.238794
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