24 April 1996 Sensor materials for an intravascular fiber optic nitric oxide sensor
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Abstract
Nitric oxide (NO) is an important regulatory molecule in physiological processes including neurotransmission and the control of blood pressure. It is produced in excess during septic shock, the profound hypotensive state which accompanies severe infections. In-vivo measurement of NO would enhance the understanding of its varied biological roles. Our goal is the development of an intravascular fiber-optic sensor for the continuous measurement of NO. This study evaluated nitric oxide sensitive compounds as potential sensing materials in the presence and absence of oxygen. Using absorption spectroscopy we studied both the Fe II and Fe III forms of three biologically active hemes known to rapidly react with NO: hemoglobin, myoglobin, and cytochrome-c. The Fe II forms of hemoglobin and myoglobin and the Fe III form of cytochrome-c were found to have the highest sensitivity to NO. Cytochrome c (Fe III) is selective for NO even at high oxygen levels, while myoglobin is selective only under normal oxygen levels. NO concentrations as low as 1 (mu) M can be detected with our fiber-optic spectrometer using cytochrome c, and as low as 300 nM using myoglobin. Either of these materials would be adequate to monitor the increase in nitric oxide production during the onset of septic shock.
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Babs R. Soller, Babs R. Soller, Bhairavi R. Parikh, Bhairavi R. Parikh, Russell F. Stahl, Russell F. Stahl, } "Sensor materials for an intravascular fiber optic nitric oxide sensor", Proc. SPIE 2676, Biomedical Sensing, Imaging, and Tracking Technologies I, (24 April 1996); doi: 10.1117/12.238800; https://doi.org/10.1117/12.238800
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