Chromosome mechanics in vivo: quantitative analysis of nonrigid 3D chromosome motion in Drosophila embryos

Wallace F. Marshall; David A. Agard; John W. Sedat

doi:10.1117/12.239542

10 May 1996 Chromosome mechanics in vivo: quantitative analysis of nonrigid 3D chromosome motion in Drosophila embryos

Wallace F. Marshall, David A. Agard, John W. Sedat

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239542
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Chromosomes are often arranged into specific configurations. One example is the metaphase plate of the Drosophila embryo in which chromosomes are arranged into a parallel bundle. How is this configuration established and maintained? Quantitative analysis of chromosomes motion in vivo should help answer this question by providing a measure of the relevant mechanical properties of the chromosomes themselves. In addition, motion analysis will allow us to study interactions of chromosomes with the mitotic spindle. In order to analyze moving mitotic chromosomes, we acquire time-lapse 3D images of chromosomes in living Drosophila embryos, and then interactively model the chromosome configuration at each time point. A model-based motion estimation algorithm is then applied. From the motion estimate, we can visualize trajectories of different regions on the chromosomes, such as centromeres and telomeres, during metaphase and during prometaphase congression. In addition, quantitative estimates of mechanical properties such as mobility and flexibility can be computed. In this preliminary report we describe computational tools for tracking and visualizing 3D chromosome motion, and for detecting oscillations in position along the mitotic spindle.

Citation Download Citation

Wallace F. Marshall, David A. Agard, and John W. Sedat "Chromosome mechanics in vivo: quantitative analysis of nonrigid 3D chromosome motion in Drosophila embryos", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239542

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available