Fluorescence lifetime imaging in cell biology

Brian Herman; Xue F. Wang; Ammasi Periasamy; Seongwook Kwon; Gerald W. Gordon; Pawel Wodnicki

doi:10.1117/12.239553

10 May 1996 Fluorescence lifetime imaging in cell biology

Brian Herman, Xue F. Wang, Ammasi Periasamy, Seongwook Kwon, Gerald W. Gordon, Pawel Wodnicki

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239553
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

The measurement of fluorescence lifetimes offers the advantages of being independent of local intensity and concentration of the fluorophore, and can provide information regarding the molecular environment in a single living cell. Historically, measurements of fluorescence lifetimes have employed photomultipliers as detectors, providing high sensitivity but sacrificing spatial information. Fluorescence Lifetime Imaging Microscopy (FLIM) provides a 2- or 3D spatial map of the distribution of fluorescent lifetime(s) in the sample under observation. Picosecond laser pulses from a tunable dye laser are delivered to fluorophore containing living cells on the stage of a fluorescent microscope, and images of the fluorescence emission at various times during the decay of the fluorescence lifetime are collected using a high speed nanosecond-gated multichannel plate image intensifier. FLIM promises to substantially enhance the information obtainable from living cells and tissues, and will allow observations of the dynamic organization and interaction of cellular components on a spatial and temporal scale previously not possible using other microscopic techniques.

Citation Download Citation

Brian Herman, Xue F. Wang, Ammasi Periasamy, Seongwook Kwon, Gerald W. Gordon, and Pawel Wodnicki "Fluorescence lifetime imaging in cell biology", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239553

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