Laser Doppler monitoring of alterations in fish embryo blood flow in response to external stimuli

Alexander V. Priezzhev; Natalia B. Savchenko; Borislav A. Levenko

doi:10.1117/12.239528

10 May 1996 Laser Doppler monitoring of alterations in fish embryo blood flow in response to external stimuli

Alexander V. Priezzhev, Natalia B. Savchenko, Borislav A. Levenko

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239528
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Noninvasive registration and adequate analysis of response of living systems to low energy physical stimuli play an important role in contemporary biological and biomedical research. In this paper we discuss the possibilities of laser Doppler microscopy (LDM) application for the study of light irradiation and thermal effects on animal organisms. Fish embryos are shown to fit many requirements related to the performance of experiments in clearly defined conditions. LDM yielding quantitative data on the dynamic response of the embryos to temperature variations and light irradiation is proved to be an adequate diagnostic technique. A fringe- mode sign-sensitive LD microscope was used to monitor the alterations of blood flow in fish embryos. Measurements were performed with spatial resolution as small as 10 micrometers in real time scale. Experimental data on the biological effects of thermal and nonthermal light stimuli on different fish species are presented.

Citation Download Citation

Alexander V. Priezzhev, Natalia B. Savchenko, and Borislav A. Levenko "Laser Doppler monitoring of alterations in fish embryo blood flow in response to external stimuli", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239528

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available